Indian Muntjac Deerskin Fibroblast Cells with DAPI, MitoTracker Red CMXRos, and Alexa Fluor 488

DAPI-FITC-TRITC Bandpass Emission (Triple Band Excitation) Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells stained with MitoTracker Red CMXRos and Alexa Fluor 488, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers, while the corresponding values for Alexa Fluor 488 are 495 and 519 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; ultraviolet excitation and blue emission). Note the presence of high signal levels from the blue (DAPI) fluorophore, as well as the orange-red fluorescence exhibited by the tubular mitochondria and the green emission from actin filaments in the cytoplasm.