Indian Muntjac Deer Skin Fibroblast Cells

The peroxisomes present in the Indian Muntjac fibroblast cell culture illustrated in the digital image above were immunofluorescently labeled with Cy2 conjugated to goat secondary antibody fragments directed against rabbit primary antibodies to peroxisomal membrane protein 70 (PMP 70), a major peroxisome membrane polypeptide. In addition, the culture was labeled for F-actin with Alexa Fluor 568 conjugated to phalloidin and for DNA with the nucleic acid stain DAPI. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.