Indian Muntjac Deer Skin Fibroblast Cells
The culture of Muntjac cells illustrated above was utilized in a double immunofluorescence experiment. The microtubule network was visualized with mouse anti-tubulin primary antibodies, while the Golgi complex was stained with rabbit anti-giantin antibodies. Secondary antibodies were goat anti-mouse and anti-rabbit conjugated to Alexa Fluor 488 and Alexa Fluor 568, respectively. Nuclei were counterstained with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.