Indian Muntjac Deer Skin Fibroblast Cells

A culture of Indian Muntjac fibroblasts (illustrated above) was immunofluorescently labeled for the microtubule network with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse secondary antibody fragments conjugated to Alexa Fluor 405. The actin cytoskeletal framework was also probed with Alexa Fluor 568 conjugated to phalloidin, and nuclei were counterstained with SYTOX Green. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.