Indian Muntjac Deer Skin Fibroblast Cells

The culture of Indian Muntjac deer skin cells depicted in the digital image above was labeled with the nucleic acid stain DAPI (nucleus) and Alexa Fluor 568 conjugated to phalloidin, which targets the F-actin cytoskeletal network. In addition, the cells were transfected with a pEYFP-Mitochondria chimeric plasmid subcellular localization vector, thus localizing a yellow fluorescent protein tag to the intracellular mitochondrial network. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.