Indian Muntjac Deer Skin Fibroblast Cells

The culture of Indian Muntjac deer skin cells presented in the digital image above was labeled with MitoTracker Red CMXRos, Alexa Fluor 350 conjugated to phalloidin, and SYTOX Green, targeting mitochondria, F-actin, and nuclear DNA, respectively. Often used in combination, these fluorophores are very popular for multi-labeling experiments to ascertain distribution of subcellular components in fixed and permeabilized cell cultures. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.