Indian Muntjac Deer Skin Fibroblast Cells
The adherent culture of Indian Muntjac deer skin cells presented in the digital image above was transfected with a pDsRed-Mitochondria plasmid subcellular localization vector, thus localizing a red fluorescent protein tag to the intracellular mitochondrial network. The culture was fixed and subsequently labeled with Hoechst 33342 and Alexa Fluor 488 conjugated to phalloidin, targeting nuclear DNA and F-actin, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.