Indian Muntjac Deer Skin Fibroblast Cells

The proximity of F-actin, mitochondria, and cell nuclei was visualized in the culture of Indian Muntjac cells shown in the digital image above with Alexa Fluor 488 conjugated to phalloidin, MitoTracker Red CMXRos, and Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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