Indian Muntjac Deer Skin Fibroblast Cells

The culture of Indian Muntjac deer skin cells presented in the digital image above was labeled with DAPI to target DNA, as well as Alexa Fluor 568 conjugated to phalloidin to target the cytoskeletal filamentous actin network. In addition, an Alexa Fluor 488-concanavalin A conjugate was used to target the endoplasmic reticulum and selected carbohydrate residues found in glycoproteins, enzymes, and cell membranes. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.