Indian Muntjac Deer Skin Fibroblast Cells
The peroxisome organelles present in the Indian Muntjac fibroblast cell culture shown above were immunofluorescently labeled with Rhodamine Red conjugated to antibodies directed against peroxisomal membrane protein 70 (PMP 70). Alexa Fluor 488 conjugated to phalloidin and DAPI were simultaneously used to counterstain the culture, targeting cytoskeletal F-actin and nuclear DNA, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.