Indian Muntjac Deer Skin Fibroblast Cells

A culture of Indian Muntjac fibroblast cells (illustrated above) was transfected with a DsRed-Mitochondria plasmid subcellular localization vector to target cellular mitochondria. Stable transfectants were isolated and grown into log phase before being fixed, permeabilized, and labeled with Hoechst 33342 and Alexa Fluor 488 conjugated to phalloidin, targeting DNA in the cell nucleus and the F-actin cytoskeletal network, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.