Indian Muntjac Nuclei

B-2E/C Bandpass Emission (Medium Bandwidth Excitation) Blue Set

Fluorescence emission intensity from a culture of Indian Muntjac cells that were labeled with SYTOX Green to stain chromatin in the nuclei. The visible light absorption maximum of SYTOX Green is 504 nanometers and the emission maximum occurs at 523 nanometers. In addition, the specimen was simultaneously labeled with primary anti-human OxPhos Complex V inhibitor protein mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Pacific Blue (absorption maximum at 410 nanometers). The actin cytoskeletal network was also labeled with phalloidin conjugated to Alexa Fluor 568. Note the absence of signals from the red (Alexa Fluor 568) and blue (Pacific Blue) fluorophores, but the presence of intense green fluorescence from the interphase cellular nuclei and nucleoli.

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