Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)
The mitochondrial network was targeted in a culture of Madin-Darby ovine kidney cells (depicted above) with MitoTracker Red CMXRos. In addition, cell nuclei were counterstained with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.