Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

The proximity of nuclei and the Golgi complex in the MDOK cell culture illustrated above was probed in a double immunofluorescence experiment with mouse anti-histones (pan) and rabbit anti-giantin primary antibodies. The antibody targets were visualized with goat secondary antibodies conjugated to Texas Red and Alexa Fluor 488, respectively. In addition, the F-actin cytoskeletal framework was labeled with Alexa Fluor 350 conjugated to phalloidin. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.