Male Rat Kangaroo Kidney Epithelial Cells (PtK2 Line)
Testing indicates that the PtK2 cell line demonstrates susceptibility to a number of different viruses, including herpes simplex, vaccinia, coxsackievirus A9, and vesicular stomatitis (Ogden strain). Poliovirus 2, adenovirus 5, and coxsackievirus B5 are among the viruses to which the cells have proven to be resistant. PtK2 epithelial cells stain positive for the intermediate filament protein keratin and are negative for the enzyme reverse transcriptase. Similar to other epithelial cells, cultured PtK2 cells exhibit significant contact inhibition to migration, tending to form close bonds with one another and forming sheets similar to the epithelial sheets that occur naturally in the body.