Male Rat Kangaroo Kidney Epithelial Cells (PtK2 Line)

The PtK2 cells presented in the digital image above were resident in a culture fluorescently labeled with Texas Red conjugated to the enzyme DNase I, Alexa Fluor 488 conjugated to phalloidin, and DAPI, targeting globular (unpolymerized) actin, the filamentous F-actin network, and nuclei, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.