Male Rat Kangaroo Kidney Epithelial Cells (PtK2 Line)

In order to visualize the relationship between the nucleus and the intracellular microtubular network, the culture of rat kangaroo kidney (PtK2) cells presented in the digital image above was immunofluorescently labeled with anti-tubulin (pan) mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Cy3. DNA in the cell nucleus was counterstained with DAPI. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.