Male Rat Kangaroo Kidney Epithelial Cells (PtK2 Line)
The rat kangaroo kidney (PtK2) cell culture depicted in the digital image above was immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse secondary antibody fragments conjugated to Rhodamine Red-X in order to target the microtubule network. The specimen was counterstained for DNA in the cell nucleus with DAPI. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.