Male Rat Kangaroo Kidney Epithelial Cells (PtK2 Line)

Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize distribution of the microtubule network in the PtK2 epithelial cell culture illustrated above. The secondary antibody (goat anti-mouse IgG) was conjugated to Cy2. Nuclei were counterstained with DAPI. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.