Mink Uterus Endometrium Epithelial Cells (GMMe Line)

The culture of mink endometrial (GMMe) cells presented in the digital image above was labeled for mitochondria and the cytoskeletal filamentous actin network with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, respectively. In addition, cell nuclei were targeted with the nucleic acid stain Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.