Mink Uterus Endometrium Fibroblast Cells (GMMs Line)

A culture of GMMs fibroblasts (featured in the digital image above) was labeled for the mitochondrial network with MitoTracker Red CMXRos, a derivative of X-rosamine. In addition, the culture was labeled for the cytoskeletal F-actin and DNA in the cell nucleus with Alexa Fluor 488 conjugated to phalloidin and Hoechst 33342, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.