Mongolian Gerbil Lung Fibroblast Cells (GeLu Line)

The GeLu cell culture presented in the digital image above was immunofluorescently labeled with mouse anti-alpha-tubulin primary antibodies followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568. In addition, the specimen was counterstained with TO-PRO-3, which targets nuclear DNA. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles with the exception of TO-PRO-3, which was pseudocolored yellow.