Mouse Hemangioendothelioma Endothelial Cells (EOMA Line)

A BODIPY FL-phalloidin conjugate was utilized to fluorescently label the cytoskeletal filamentous actin network of the EOMA endothelial cell culture presented in the digital image above. The cells were also treated with the probes MitoTracker Orange CMTMRos and Hoechst 33342 to target mitochondria and DNA in the cell nucleus, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.