Mouse Kidney Tissue with Alexa Fluor 488 and 568

FITC-Texas Red Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a thin section of mouse kidney stained with multiple (3) fluorophores. Nuclei in the tissue section were targeted with the nucleic acid probe DAPI, which has an excitation maximum at 358 nanometers and an emission maximum at 461 nanometers when bound to DNA in cell cultures and tissue sections. In addition, the cryostat section was also simultaneously stained with Alexa Fluor 488 wheat germ agglutinin (glomeruli and convoluted tubules; 519-nanometer emission) and Alexa Fluor 568 phalloidin (filamentous actin and the brush border). Note the presence of signal from both the green (Alexa Fluor 488) and red (Alexa Fluor 568) probes, but the absence of blue fluorescence due to DAPI, which is suppressed by the Nikon FITC-Texas Red filter combination.