Normal African Green Monkey Kidney Fibroblast Cells (CV-1 Line)
In order to visualize lectin binding to the Golgi complex in CV-1 cells, the adherent culture illustrated above was treated with wheat germ agglutinin conjugated to Oregon Green 488. The cells were subsequently counterstained with Alexa Fluor 568 conjugated to phalloidin to localize the filamentous actin network, and the nucleic acid stain DAPI to label DNA in the nucleus. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.