Normal African Green Monkey Kidney Fibroblast Cells (CV-1 Line)

The mitochondria present in the culture of monkey kidney cells (CV-1 line) featured in the digital image above were fluorescently labeled with MitoTracker Red CMXRos, a derivative of X-rosamine. In addition, the culture was labeled for the cytoskeletal F-actin network and DNA in the cell nucleus with Alexa Fluor 633 conjugated to phalloidin and SYTOX Green, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles with the exception of Alexa Fluor 633, which was pseudocolored blue.