Normal African Green Monkey Kidney Fibroblast Cells (CV-1 Line)
The culture of African green monkey kidney cells presented in the digital image above was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse secondary antibody fragments (Fab) conjugated to Rhodamine Red-X. In addition, the specimen was subsequently counterstained for DNA in the cell nucleus with DAPI. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.