Normal Human Lung Cells with Cy2, DAPI, and Alexa Fluor 546
DAPI-FITC-TRITC Bandpass Emission (Triple Band Excitation) Set
Fluorescence emission intensity from a culture of normal human lung cells (MRC-5 line) that were immunofluorescently labeled with primary anti-ATPase inhibitor protein mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy2. The visible light absorption maximum of Cy2 is 489 nanometers and the emission maximum occurs at 506 nanometers in the blue-green region of the spectrum. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and Alexa Fluor 546 conjugated to phalloidin (targeting the cytoskeletal actin network; yellow-orange emission). Although not formally considered a member of the mitochondrial Complex V protein network, the ATPase inhibitor protein has been demonstrated to associate with this oxidative phosphorylation system. Note the presence of green mitochondria in this specimen.