Opossum Kidney Cortex Epithelial Cells (OK Line)

The opossum kidney epithelial cells presented in the digital image above were immunofluorescently labeled with mouse anti-alpha-tubulin primary antibodies followed by goat anti-mouse secondary antibodies conjugated to Marina Blue. Tubulin is the basic component of microtubules. In addition, the specimen was stained with SYTOX Green to target DNA. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.