Opossum Kidney Cortex Epithelial Cells (OK Line)

The adherent culture of opossum kidney epithelial cells (OK line) featured in the digital image above was transfected with pDsRed2-Mitochondria, pEGFP-Tubulin, and pECFP-Nucleus plasmid subcellular localization vectors, thus localizing a red fluorescent protein tag to the intracellular mitochondrial network, a green tag to microtubules, and a cyan protein tag to cell nuclei. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.