Opossum Kidney Cortex Epithelial Cells (OK Line)

Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize distribution of microtubules in the log phase monolayer culture of opossum kidney cells presented above. The secondary antibody (goat anti-mouse IgG) utilized was conjugated to Marina Blue. DNA in the cell nucleus was counterstained with SYTOX Green. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.