Opossum Kidney Cortex Epithelial Cells (OK Line)

Three subcellular localization vectors were utilized to target enhanced fluorescent proteins directly into organelles present in a culture of opossum kidney cells. Use of pDsRed2-Mitochondria, pEGFP-Tubulin, and pECFP-Nucleus plasmid vectors enabled the localization of a red fluorescent protein tag to mitochondria, a green tag to microtubules, and a cyan protein tag to cell nuclei. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.