Rabbit Kidney Epithelial Cells (RK13 Line)
The culture of rabbit kidney epithelial cells presented in the digital image above was labeled for the intracellular mitochondrial network with MitoTracker Red CMXRos. DNA in the cell nucleus was counterstained with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.