Rabbit Lung Tissue
FITC-Texas Red Bandpass Emission (Dual Band Excitation) Set
Fluorescence emission intensity from a thin section of rabbit lung tissue stained for actin filaments and glycoproteins with Alexa Fluor 546-phalloidin and Oregon Green 488 wheat germ agglutinin, respectively. The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers, while the corresponding values for Oregon Green 488 are 496 and 524 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the nucleus; blue emission). Note the absence of signal from the blue fluorophore, but the bright orange-red fluorescence exhibited by filamentous actin and the green emission from glycoproteins in the cytoplasm and membrane components of the tissue section.