Rat Jejunum Myenteric Plexus Enteroglial Cells (EGC/PK060399egfr Line)

The popular triple fluorophore combination of MitoTracker Red CMXRos, BODIPY FL conjugated to phalloidin, and Hoechst 33258 was used to label the adherent log phase culture of rat jejunum enteroglial cells illustrated above for mitochondria, the filamentous actin network, and nuclear DNA. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.