Rat Jejunum Myenteric Plexus Enteroglial Cells (EGC/PK060399egfr Line)
The adherent culture of EGC cells illustrated above was labeled with Alexa Fluor 488 conjugated to soybean agglutinin, a lectin isolated from Glycine max that has saccharide binding sites that recognize and bind terminal alpha- and beta-N-acetylgalactosamine and galactopyranosyl oligosaccharides. In addition, the cells were labeled with Alexa Fluor 568 conjugated to phalloidin and DAPI, targeting the cytoskeletal F-actin network and DNA, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.