Rat Jejunum Myenteric Plexus Enteroglial Cells (EGC/PK060399egfr Line)

The culture of EGC cells presented in the digital image above was triple-labeled for mitochondria, filamentous actin, and nuclear DNA with MitoTracker Red CMXRos, BODIPY FL conjugated to phalloidin, and Hoechst 33258, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.