Rat Jejunum Myenteric Plexus Enteroglial Cells (EGC/PK060399egfr Line)
The cytoskeletal filamentous actin network was labeled in the culture of EGC cells presented in the digital image above with Coumarin conjugated to phalloidin (yielding blue emission). Mitochondria and nuclei were also visualized in the cells with MitoTracker Red CMXRos and SYTOX Green, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.