Rat Kangaroo Cellular Vimentin Intermediate Filament Network with Rhodamine Red

TRITC HYQ Bandpass Emission (Medium Bandwidth Excitation) Green Set

Fluorescence emission intensity from a culture of rat kangaroo kidney epithelial cells (PtK2line) that were immunofluorescently labeled with primary anti-vimentin (an intermediate filament protein) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Rhodamine Red. The visible light absorption maximum of Rhodamine Red is 570 nanometers and the emission maximum occurs at 590 nanometers. In addition, the specimen was stained with DAPI (targeting DNA in the nucleus; ultraviolet excitation and blue emission), but this fluorophore is not imaged with the Nikon TRITC HYQ filter combination. Note the bright fluorescence intensity exhibited by the vimentin intermediate filament network, which extends throughout the cytoplasm in these epithelial cells.