Rat Throacic Aorta Cellular Mitochondria

Texas Red HYQ Bandpass Emission (Wide Bandwidth Excitation) Yellow Set

Fluorescence emission intensity from a culture of rat thoracic aorta (smooth muscle) cells stained with MitoTracker Red CMXRos, which targets the intracellular mitochondrial network. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and BODIPY FL-phallacidin (targeting actin; green emission). Note the absence of signal from the blue (DAPI) and green (BODIPY) fluorophores, but the bright red fluorescence exhibited by the tubular mitochondria.