Rat Tongue Muscle Tissue with Rhodamine, DAPI, and Alexa Fluor 488

DAPI-FITC-TRITC Bandpass Emission (Triple Band Excitation) Set

Fluorescence emission intensity from a thin section of rat tongue muscle tissue stained for DNA and glycoproteins with DAPI and tetramethylrhodamine (TMR) conjugated to wheat germ agglutinin, respectively. The ultraviolet light absorption maximum of DAPI is 358 nanometers and the visible emission maximum occurs at 461 nanometers, while the corresponding values for TMR are 550 and 573 nanometers. In addition, the specimen was simultaneously labeled with Alexa Fluor 488 conjugated to phalloidin (targeting the actin network; green emission). Note the presence of both overlapping and discrete signals from all three of these fluorophores, which combine to form an image reminiscent of a brightfield stain.

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