Rhesus Monkey Kidney Epithelial Cells (LLC-MK2 Line)

A culture of LLC-MK2 monkey kidney cells (depicted above) was fluorescently labeled for the mitochondrial network with MitoTracker Red CMXRos, a derivative of X-rosamine. In addition, the culture was stained with Hoechst 33258, targeting DNA in the cell nucleus. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.