Swiss Mouse Embryo Moloney Murine Leukemia Virus Transfected Fibroblast Cells (CRE BAG 2 Line)

The adherent culture of CRE BAG 2 fibroblast cells featured in the digital image above was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which bind with mitochondria, F-actin, and nuclear DNA, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.