Tahr Ovary Epithelial Cells (HJ1.Ov Line)
The relationship between the mitochondrial and filamentous actin networks was visualized in a culture of HJ1.Ov cell (depicted above) with MitoTracker Red CMXRos and Oregon Green 488 conjugated to phalloidin. The culture was counterstained for DNA in the cell nucleus with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.