Tahr Ovary Epithelial Cells (HJ1.Ov Line)
The HJ1.Ov cell culture featured above was fixed, permeabilized, washed, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with rabbit primary antibodies to giantin, a protein resident in the Golgi complex of mammalian cells. The culture was subsequently stained with a mixture of secondary antibodies conjugated to Alexa Fluor 568. In addition, nuclear pore complexes were immunofluorescently labeled with primary anti-NPCP mouse monoclonal antibodies followed by goat anti-mouse Fab heavy and light chain fragments conjugated to Alexa Fluor 488. The F-actin actin network was counterstained with Alexa Fluor 350 conjugated to phalloidin. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.