Tahr Ovary Epithelial Cells (HJ1.Ov Line)

A culture of HJ1.OV epithelial cells (depicted above) was immunofluorescently labeled for the nuclear pore and Golgi complexes with a cocktail of mouse anti-NPCP (nuclear pore complex protein) and rabbit anti-giantin primary antibodies followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 488 and Alexa Fluor 568, respectively. The actin cytoskeleton was targeted with Alexa Fluor 350 conjugated to phalloidin. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.