Transformed African Green Monkey Kidney Cell Actin
YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set
Fluorescence emission intensity from a culture of transformed (SV-40) African green monkey kidney fibroblast cells (COS-7 line) that were transfected with a pEYFP-Actin plasmid subcellular localization vector. Cells were transiently transfected and cultured in nutrient medium for a minimum of 24 hours before recording images. The enhanced yellow fluorescent protein gene used in these studies contains several important amino acid substitutions that shift the emission maximum of green fluorescent protein (GFP) by approximately 18 nanometers, from 509 to 527 nanometers. However, the fluorescence emission still appears green when observed in the microscope eyepieces. Note the sparse network of actin filaments and stress fibers that extend across the cell, but the high degree of three-dimensional gel-like character exhibited by actin fibers throughout the cytoplasm and cortex in this cell line.