Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-1 Line)
The intracellular relationship between mitochondria and the filamentous actin network was visualized in a culture of COS-1 fibroblasts (illustrated above) with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin. Cell nuclei were counterstained with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.