Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-1 Line)

The transformed African green monkey kidney cells presented in the digital image above were resident in an adherent culture immunofluorescently labeled with Alexa Fluor 488 and Alexa Fluor 568 conjugated to goat secondary antibodies that target mouse anti-fibrillarin (nucleoli) and rabbit anti-giantin (targeting the Golgi complex) primary antibodies, respectively. Nuclei were stained with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.