Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-7 Line)
The COS-7 fibroblast cells presented in the digital image above were resident in an adherent culture stained for F-actin with Alexa Fluor 546 conjugated to phalloidin, and for DNA with SYTOX Green. The mitochondrial network was simultaneously visualized using MitoTracker Deep Red 633. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles with the exception of MitoTracker Deep Red 633, which was pseudocolored yellow.